The United States imports approximately 32% of fresh vegetables and 55% of fresh fruits annually for domestic consumption; however, formal training on the global fresh produce supply chain remains limited in many higher education agricultural programs. To address this gap, a Global Fresh Produce Market course was developed for junior- and senior-level students that integrated lectures, laboratory activities, industry visits, and an international field experience to reinforce classroom concepts and connect theory to practice. Lecture content was intentionally aligned with visits to ag-related industries along the U.S.-Mexico border. Laboratory exercises focused on fruit quality assessment, food safety, control of invasive pests and biosecurity. Students toured packing houses and distribution centers to observe the application of quality assurance and control procedures during receiving operations and to examine preventive practices designed to reduce the risk of foodborne illness in fresh produce. Visits to USDA Animal and Plant Health Inspection Service (APHIS) facilities highlighted inspection protocols at ports of entry and the role of regulatory agencies in preventing the introduction of restricted materials, pests, and pathogens into the U.S. Students also engaged with the USDA Plant Protection and Quarantine fruit fly eradication program, gaining insight into sterile insect release strategies and the scale of effort required to manage invasive pests. Learning outcomes were further reinforced through an international visit to certified greenhouse operations in Mexico, where students observed strict sanitary protocols for production and shipment into the U.S. Student surveys indicated an increase in knowledge across all course topics and high levels of perceived learning value. A student-centered mentorship component was also incorporated to enhance engagement, support retention, and inform career decision-making. This multi-component program enhanced student understanding of the global food supply chain and strengthened their ability to connect theory, practice, and policy to support the safety and sustainability of U.S. agriculture.