The fashion industry is one of the most environmentally contaminating industries, contributing to pollution and microplastic release associated with synthetic fibers. In response, the industry is adopting sustainable practices and revisiting renewable and biodegradable fibers including wool and cotton. Education and literacy surrounding sustainable fiber and textile practices are fundamental for preparing a future workforce. Agricultural and fashion educators play a critical role in highlighting emerging opportunities within the industry and in shaping how students broadly conceptualize the purpose and process behind fiber and textile production. Undergraduates enrolled in three agriculture (AELC and AGS) and fashion design (FDM) programs were surveyed to determine their perceived knowledge of agricultural production and natural fiber systems, specifically wool. The questionnaire consisted of 25 multiple-choice, short-answer, and Likert-type items related to agricultural literacy and concepts in fiber production. Seventy-three shared (n=73) their understanding of natural fiber sources, identification, properties, common misconceptions, and sustainability attributes. The sample included 37.0% (n=27) FDM, 53.4% (n=39) AELC, and 9.6% (n=7) were AGS majors. Respondents had a moderate to high understanding of natural and synthetic fibers, but a low to moderate grasp of their physical properties. However, they demonstrated a moderate to high awareness of the importance of sustainable natural fibers. A series of Kruskal–Wallis H tests and post–hoc pairwise comparisons showed that FDM students reported statistically significantly higher confidence in their knowledge of natural fibers. FDM and AELC understand wool properties significantly more than AGS students. However, FDM and AGS value this knowledge in their field (p <.001) indicating a stronger foundation in fiber-related concepts. However, deviation in responses across majors revealed gaps in knowledge of production and fiber literacy. These findings highlight opportunities for agricultural and fashion educators to collaborate to develop interconnected curriculum for production and byproducts, including textile fibers and apparel manufacturing.