Purpose
Effective communication between patients and physicians is an essential skill that must be honed over time. The development of these skills is fostered throughout medical education, in part, by initiatives aimed at teaching K-12 students. The current work is founded on outreach educational interactions led by osteopathic medical students (OMS), who use active learning and clinical cases to teach biochemistry concepts to high school students (HSS). Aim: to determine if the OMS could effectively teach biochemistry concepts to HSS by assessing knowledge acquisition from the teaching session. Hypothesis: Advanced Placement (AP) biology students will learn biochemistry concepts to a greater degree than Traditional biology students due to their higher base knowledge.

Methods
Two groups of HSS were assessed: AP Biology students (n=19) and Traditional Biology students (n=11). Identical procedures were implemented in both groups using a five multiple-choice questions (MCQ) pre-quiz, an active learning activity, a 30-minute presentation on the biochemical processes of diabetes, and a five-MCQ post-quiz featuring case-based vignettes aligned with the MCQ in the pre-quiz.

Results
Statistically significant improvements were observed in the pre-versus post-quiz scores of AP students in 2 out of the 5 questions. For pre-quiz question 2, aligning with post-quiz question 5, AP students improved from 15.79% to 63.16% of correctness (p-value=0.014). Similarly, for pre-quiz question 3 and post-quiz question 2, students improved from 21.05% to 73.66% (p-value=0.006). In contrast, data collected from Traditional students did not show statistically significant improvement (p-value >0.05) in percent correctness between the pre-and post-quiz.

Conclusion
The results of this study are significant, as they demonstrate that, following the presentation taught by OMS, AP students learned and applied biochemical concepts better than Traditional students. This finding underscores the potential of medical students to enhance their understanding of biochemistry among high school students.